Related Experiment Video
Updated: Jul 13, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
Published on: June 17, 2019
Inferring the evolutionary history of vibrios by means of multilocus sequence analysis
Tomoo Sawabe1, Kumiko Kita-Tsukamoto, Fabiano L Thompson
1Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041-8611, Japan. sawabe@fish.hokudai.ac.jp
Abstract:
We performed the first broad study aiming at the reconstruction of the evolutionary history of vibrios by means of multilocus sequence analysis of nine genes. Overall, 14 distinct clades were recognized using the SplitsTree decomposition method. Some of these clades may correspond to families, e.g., the clades Salinivibrio and Photobacteria, while other clades, e.g., Splendidus and Harveyi, correspond to genera. The common ancestor of all vibrios was estimated to have been present 600 million years ago. We can define species of vibrios as groups of strains that share >95% gene sequence similarity and >99.4% amino acid identity based on the eight protein-coding housekeeping genes. The gene sequence data were used to refine the standard online electronic taxonomic scheme for vibrios (http://www.taxvibrio.lncc.br).
Related Concept Videos
Modern Molecular Taxonomy
Evolutionary Relationships through Genome Comparisons
Microbial Phylogeny
Applications of Molecular Taxonomy
Phylogenetic Species Concept in Microbiology
Evolution of Microbial Genome

