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Updated: Jul 13, 2026

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug
Published on: December 14, 2021
One-step method for catheterising the jugular vein of cats to enable repeated blood sampling
C Lainesse1, S R Boysen, M Y Doucet
1Départment de Biomédicine Vétérinaire, Université de Montréal, CP 5000, St-Hyacinthe, Quebec J2S 7C6, Canada.
Insights
A new one-step jugular vein catheterization method in cats allows for multiple blood samples. While haematocrit and total solids decreased, no significant clinical issues were observed in feline patients.
Area of Science:
- Veterinary Medicine
- Animal Science
- Surgical Techniques
Background:
- Jugular vein catheterization is crucial for diagnostics in cats.
- Previous methods may be invasive or less efficient for serial sampling.
- Optimizing catheter length is essential for patient safety and sample quality.
Purpose of the Study:
- To develop and evaluate a one-step method for jugular vein catheterization in cats.
- To determine the optimal internal catheter length for adult cats using radiography.
- To assess the impact of serial blood sampling and heparin flushes on feline haematocrit and total solids.
Main Methods:
- A novel one-step catheterization technique was employed for feline jugular veins.
- Radiography was utilized to ascertain appropriate internal catheter dimensions.
- Serial blood samples were collected over 48-hour periods, with intermittent heparin flushes.
- Haematocrit and total solids were measured to evaluate physiological effects.
Main Results:
- A 97% success rate was achieved in collecting blood samples using a 19 G, 30.5 cm catheter.
- The catheter was successfully maintained in situ for two 48-hour periods.
- Significant decreases in haematocrit and total solids were noted in all cats.
- No clinically significant blood loss or coagulation abnormalities were detected.
Conclusions:
- The developed one-step jugular catheterization is an effective method for serial blood sampling in cats.
- The technique is safe, with minimal adverse effects on feline haematological parameters.
- This method provides a reliable approach for intensive monitoring and research in feline medicine.
Abstract:
A one-step method for catheterising the jugular vein of cats for taking multiple blood samples was developed, with the aid of radiography, to determine an appropriate internal catheter length for adult cats. The effects of multiple blood sampling and heparin flushes on the cats' haematocrit and blood total solids were also assessed. Seven healthy adult cats were used. A total of 128 of 132 (97 per cent) blood samples were collected successfully through a 19 G, 30.5 cm catheter introduced as a central venous catheter and maintained in place during two periods of 48 hours. The haematocrit and total solids were significantly decreased in all the cats, but no clinically significant blood loss or coagulation disorders were observed.
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