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Updated: Jul 13, 2026

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Published on: February 24, 2014
Highly efficient somatic-mutation identification using Escherichia coli mismatch-repair detection
Brock A Peters1, Zhengyan Kan, Dragan Sebisanovic
1Department of Molecular Biology, Genentech, 1 DNA Way, South San Francisco, California 94080, USA.
Abstract:
The discovery of somatic mutations in cancer tissue is extremely laborious, time-consuming and costly. In an evaluation comparing mismatch repair detection (MRD) against Sanger sequencing for somatic-mutation detection, we found that MRD had a specificity of 96% and a sensitivity of 92%. Our results showed that MRD is a robust and cost-effective alternative to Sanger sequencing for identifying somatic mutations in human tumors.
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