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Updated: Jul 13, 2026
Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
Immune evaluation of biomaterials in TNF-alpha and IL-1beta at mRNA level
Tingting Ding1, Jiao Sun, Ping Zhang
1Ninth People's Hospital School of Medicine, Shanghai Jiao tong University/Shanghai Biomaterials Research & Testing Center, Shanghai 200023, China.
Objective:
To examine biomaterial biocompatibility and improve current methods of immunological evaluation TNF-alpha and IL1-beta were used as indicators at mRNA levels.
Methods:
Rat peritoneal macrophages were stimulated with different biomaterials and expression of TNF-alpha and IL1-beta was measured by RT-PCR and compared within different groups.
Results:
Macrophages that were stimulated with PTFE produced significantly more TNF-alpha and IL1-beta than unstimulated macrophages (p < 0.001). The PLGA, NPG, beta-TCP, and CPC groups also induced moderate TNF-alpha and IL1-beta expression (p < 0.01).
Conclusions:
TNF-alpha and IL1-beta are sensitive indicators of immune stimulation that can help to monitor the levels of cellular activation induced by different biomaterials. Our findings showed that beta-TCP and CPC had a good biocompability, and CPC was the most biocompatible of all the biomaterials tested. While PTFE and NPG still had side effect because of producing pro-inflammatory cytokines in vitro once implanted.
