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Related Experiment Videos

Proteomic analysis of hepatitis B surface antigen positive transgenic mouse liver and decrease of cyclophilin A.

Chao Zhao1, Cai-Yun Fang, Xiao-Chen Tian

  • 1Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, PR China.

Journal of Medical Virology
|August 21, 2007
PubMed
Summary
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Hepatitis B surface antigen (HBsAg) expression in mice alters liver protein profiles, notably decreasing cyclophilin A (CypA) within cells and increasing its secretion. This suggests HBsAg impacts cellular metabolism and protein handling.

Area of Science:

  • Hepatology
  • Virology
  • Proteomics

Background:

  • Hepatitis B virus (HBV) infection is linked to small, spherical particles composed of hepatitis B surface antigen (HBsAg).
  • These HBsAg particles often exceed the number of HBV virions, suggesting a significant role in infection dynamics.

Purpose of the Study:

  • To investigate the pathogenic interactions between liver cells and HBsAg expression.
  • To compare global protein profiles in HBsAg-positive versus HBsAg-negative mouse livers.

Main Methods:

  • Two-dimensional gel-based differential proteomics was employed.
  • Protein profiles of HBsAg-positive transgenic mice were compared to HBsAg-negative control siblings.

Main Results:

  • 93 differentially expressed proteins were identified in transgenic mice.

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  • Approximately 45% of these proteins were metabolic enzymes, indicating altered lipid, carbohydrate, and amino acid processing.
  • Cyclophilin A (CypA) was decreased intracellularly in HBsAg-positive livers and cell lines, with increased secretion into the supernatant.

    • Conclusions:

    • HBsAg expression significantly impacts cellular metabolism and protein regulation in the liver.
    • The observed decrease in intracellular CypA and its subsequent secretion may have implications for hepatitis B pathogenesis.
    • Further research is warranted to explore the functional consequences of these proteomic changes.