Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mesenchymal stem cell-derived small extracellular vesicles suppress pyroptosis by delivering miR-125a-5p to improve acute kidney injury in sepsis.

Cell death discovery·2026

Same author

Uromodulin p.His36Tyr promotes macrophage pyroptosis via App-Cd74 signaling to drive renal inflammation in ADTKD.

Nature communications·2026

Same author

Exosomal hsa-miR-299-3p from endothelium mediates high phosphorus-induced vascular calcification in mice model of CKD via phosphorylated JAK2/STAT5 pathway.

Frontiers in pharmacology·2026

Same author

Current understanding of sodium-glucose transporter 2 inhibitors in cardiovascular-kidney-metabolic syndrome.

Frontiers in pharmacology·2026

Same author

T Cell-Independent Role of PD-L1 in Kidney Repair: Mitigation of Tubular DNA Damage via PD-L1/BRCA1 Interaction Following AKI.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Shedding light on the role of extracellular vesicles in renal fibrosis.

Fundamental research·2026

Same journal

Unveiling the post translational modification code in Alzheimer's pathogenesis: crosstalk between phosphorylation and acetylation.

Expert review of proteomics·2026

Same journal

Plasma proteome profiles of standard-risk and high-risk childhood acute lymphoblastic leukemia.

Expert review of proteomics·2026

Same journal

Potential proteomic biomarkers for monitoring clinical studies in Duchenne/Becker muscular dystrophy.

Expert review of proteomics·2026

Same journal

Proteomics-based approaches to neutrophil biology.

Expert review of proteomics·2026

Same journal

Deciphering mitochondrial metabolic vulnerabilities in ovarian clear cell carcinoma with mass spectrometry-based clinical proteomics.

Expert review of proteomics·2026

Same journal

The proteomic origin of the genetic code.

Expert review of proteomics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 25, 2025

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

High-throughput antibody microarrays for quantitative proteomic analysis.

Lin-Li Lv¹, Bi-Cheng Liu

¹Institute of Nephrology, Zhong Da Hospital, Southeast University, Nanjing, China. lvll46@gmail.com

Expert Review of Proteomics

|August 21, 2007

Summary

This summary is machine-generated.

Antibody microarrays offer robust, high-throughput protein quantification for disease proteomics research. This review covers their production, application in biomarker discovery, and analytical challenges.

More Related Videos

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Related Experiment Videos

Last Updated: Dec 25, 2025

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Area of Science:

Proteomics
Biotechnology
Molecular Biology

Background:

Antibody microarrays are established tools for high-throughput, parallel measurement of known proteins.
They complement traditional proteomics methods like mass spectrometry.
Their quantitative nature makes them valuable in various research applications.

Purpose of the Study:

To review the technical aspects of antibody microarray production.
To highlight applications in quantitative proteomic analysis, particularly for disease proteomics and biomarker identification.
To discuss quality control, data analysis, and challenges in quantitative assays.

Main Methods:

Review of existing literature on antibody microarray technology.
Summarization of production methodologies.
Analysis of application data in disease proteomics.

Main Results:

Antibody microarrays provide a robust and quantitative platform for targeted proteomics.
They are effective in identifying potential protein biomarkers for various diseases.
Key considerations for quality control and data analysis are outlined.

Conclusions:

Antibody microarrays are a vital tool for quantitative proteomics and biomarker discovery.
Further development in quality control and data analysis will enhance their utility.
They offer a complementary approach to unbiased proteomics strategies.