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Updated: Jul 13, 2026
06:46
A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
Published on: July 5, 2022
Speeding up coeliac disease diagnosis in the developing countries
S Crovella1, L Brandao, R Guimaraes
1Department of Reproductive and Developmental Sciences, University of Trieste, Institute of Child Health I.R.C.C.S. Burlo Garofolo, Italy.
Summary
Rapid tests for anti-transglutaminase antibodies accurately identified coeliac disease in Brazil. These cost-effective assays are suitable for non-specialized use in developing countries, enabling wider screening for this condition.
Area of Science:
- Immunology
- Gastroenterology
- Public Health
Background:
- Anti-transglutaminase antibodies are key biomarkers for active coeliac disease.
- Limited access to antibody assays in developing nations hinders coeliac disease screening.
- Economical and accessible diagnostic tools are crucial for early detection.
Purpose of the Study:
- To assess coeliac disease prevalence in urban and impoverished sub-urban populations in Recife, Brazil.
- To evaluate the efficacy of two rapid anti-transglutaminase antibody tests using serum and whole blood.
- To determine the feasibility of these rapid tests for coeliac disease screening in resource-limited settings.
Main Methods:
- Analyzed 1074 individuals (university students and residents of impoverished areas) using two rapid anti-transglutaminase antibody tests.
- Confirmed positive rapid test results with anti-transglutaminase enzyme-linked immunosorbent assay (ELISA), HLA DQ2/8 typing, and intestinal biopsy.
- Compared test performance in urban versus sub-urban impoverished populations.
Main Results:
- Both rapid assays detected 25 positive cases out of 1074 participants.
- ELISA confirmed 9 cases (0.84% prevalence) with typical coeliac disease lesions.
- Seven confirmed coeliac disease patients were asymptomatic; two had recurrent abdominal pain.
Conclusions:
- Rapid anti-transglutaminase antibody assays are accurate and cost-effective for identifying coeliac disease.
- These tests demonstrate high potential for use by non-specialized personnel in developing countries.
- The study supports the implementation of these rapid assays for widespread coeliac disease screening in resource-limited settings.
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