Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Procollagenase from bovine gingiva.

H Birkedal-Hansen, C M Cobb, R E Taylor

    Biochimica Et Biophysica Acta
    |March 11, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Prevalence of diabetes among patients diagnosed with periodontitis: A retrospective cross-sectional study.

    International journal of dental hygiene·2017
    Same author

    Interproximal cervical lesions caused by incorrect flossing technique.

    International journal of dental hygiene·2011
    Same author

    Collagenolytic activity of synovium in rheumatoid arthritis.

    The New England journal of medicine·2010
    Same author

    Inhibition of molar eruption and root elongation in MT1-MMP-deficient mice.

    Connective tissue research·2003
    Same author

    Proton radiotherapy for paediatric tumours: potential areas for clinical research.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2003
    Same author

    DRD4 promoter SNPs and gender effects on Extraversion in African Americans.

    Molecular psychiatry·2002

    Bovine gingival explants release collagenase (EC 3.4.24.3) as an inactive zymogen. Trypsin activation converts this zymogen into active collagenase, enabling collagen hydrolysis and alpha2-macroglobulin complex formation.

    Area of Science:

    • Biochemistry
    • Enzymology
    • Connective Tissue Research

    Background:

    • Collagenase is crucial for extracellular matrix remodeling.
    • Understanding collagenase activation is vital for tissue homeostasis and disease research.
    • Bovine gingival tissue is a relevant model for studying collagenase activity.

    Purpose of the Study:

    • To characterize the zymogen form of collagenase released from bovine gingival explants.
    • To investigate the activation process of collagenase zymogen.
    • To examine the properties of both the zymogen and activated collagenase.

    Main Methods:

    • In vitro culture of bovine gingival explants.
    • Gel filtration chromatography to determine molecular weight.
    • Enzymatic assays to assess collagen hydrolysis.

    Related Experiment Videos

  • Complex formation studies with alpha2-macroglobulin (alpha2-M).
  • Limited proteolysis using trypsin for enzyme activation.

    • Main Results:

    • Collagenase is secreted as an inactive zymogen (approx. 80,000 Da) that does not degrade collagen or bind alpha2-M.
    • Trypsin activation reduces the zymogen's molecular weight by 15,000-20,000 Da, yielding active collagenase.
    • Activated collagenase can hydrolyze collagen and form a complex with alpha2-M.
    • The zymogen can be separated from the active enzyme and alpha2-M complex.
    • The zymogen can be isolated from serum-supplemented cultures.

    Conclusions:

    • Bovine gingival collagenase is synthesized and released as a latent zymogen.
    • Activation by trypsin converts the zymogen to an active enzyme with altered molecular weight and biochemical properties.
    • The findings provide insights into collagenase regulation and potential therapeutic targets in conditions involving matrix degradation.