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Updated: Jul 13, 2026
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
Evaluation of oxidative stress markers after vaginal delivery or Caesarean section
Zsuzsanna Hracsko1, Zsolt Safar, Hajnalka Orvos
1Department of Biochemistry and Molecular Biology, University of Szeged, H-6701 Szeged, Hungary. hracsko@bio.u-szeged.hu
Background:
The effects of vaginal delivery (VD) and of cesarean section (CS) on the markers of oxidative stress were investigated.
Materials And Methods:
Umbilical blood samples were analyzed from 74 full-term neonates, 46 born via VD, 28 via elective CS. The level of lipid peroxidation (LP), protein and DNA damage and the antioxidant status were compared.
Results:
Differences between CS and VD groups were generally non-significant for oxidative markers, except for the GSH concentrations (VD: 4.18 vs. CS: 2.77 microM/mg protein x 10(-3); p<0.05). LP was significantly higher in the CS group (0.078 vs. 0.042 nM MDA/mg protein; p <0.05). The level of carbonyl proteins was high in the VD group and significantly lower in the elective CS group (9.5 vs. 8.1 mM/mg protein x 10(-4); p<0.05). We found 0.78% more strand breaks in elective CS group than in VD group.
Conclusion:
CS does not have an advantage over VD with respect to oxidative stress.
