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Related Experiment Videos

Antibody screening database for protein kinetic modeling.

Brett Spurrier1, Frank L Washburn, Samuel Asin

  • 1Molecular Translational Technology Section, Molecular Therapeutics Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

Proteomics
|August 22, 2007
PubMed
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Researchers created a quality-controlled antibody database by screening 279 antibodies. This resource aids reliable proteomic studies and protein kinetic modeling using protein arrays.

Area of Science:

  • Proteomics
  • Antibody validation
  • Biotechnology

Background:

  • High-quality antibodies are essential for knowledge-based proteomic studies.
  • Commercial antibody performance varies significantly across different experimental conditions (cell types, treatments, time points).

Purpose of the Study:

  • To establish a comprehensive antibody database with validated antibodies for proteomic applications.
  • To assess antibody performance across diverse biological contexts.

Main Methods:

  • Screened 279 antibodies against multiple cell lysates.
  • Utilized various treatments and time points during antibody screening.
  • Applied quality-confirmed antibodies on protein arrays.

Main Results:

Related Experiment Videos

  • Developed a database of validated antibodies.
  • Demonstrated the utility of these antibodies for protein kinetic modeling.
  • Showcased antibody performance consistency across different experimental conditions.

Conclusions:

  • The antibody database provides a reliable resource for proteomic research.
  • Validated antibodies are crucial for accurate protein kinetic modeling.
  • Systematic antibody screening enhances data reproducibility in proteomics.