Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards robot-assisted magnetic particle imaging for mobile point-of-care (neuro)vascular applications.

Scientific reports·2026
Same author

Eye-hand coordination during robotic prostate cancer surgery - how surgical vision reflects on instrument kinematics and vice versa.

Journal of robotic surgery·2026
Same author

Surgical innovation and technology.

The British journal of surgery·2026
Same author

Short-chain fatty acids mitigate inflammation and associated metabolic programming of human stem cell-derived enteric glial cells.

Journal of neuroinflammation·2026
Same author

Structure-Activity Relationship of Cyanine Dyes in Relation to the Hepatic Uptake and Excretion.

Chembiochem : a European journal of chemical biology·2026
Same author

Robust Bioconjugated Antigens Induce Immune Responses Preventing Malaria Infection and its Transmission.

Small (Weinheim an der Bergstrasse, Germany)·2026

Related Experiment Video

Updated: Jul 13, 2026

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
10:25

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

Published on: November 10, 2012

Profiling proteasome activity in tissue with fluorescent probes.

Celia R Berkers1, Fijs W B van Leeuwen, Tom A Groothuis

  • 1Division of Cellular Biochemistry, Tumor Biology, and Immunology, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.

Molecular Pharmaceutics
|August 22, 2007
PubMed
Summary

New fluorescent probes enable sensitive profiling of proteasome activity in living cells. These tools aid in evaluating proteasome inhibitor efficacy for cancer treatment and understanding organ-specific proteasome regulation.

More Related Videos

Detection of Protease Activity by Fluorescent Peptide Zymography
09:56

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Related Experiment Videos

Last Updated: Jul 13, 2026

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
10:25

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

Published on: November 10, 2012

Detection of Protease Activity by Fluorescent Peptide Zymography
09:56

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Proteasome inhibitors are crucial in treating multiple myeloma and other cancers.
  • Accurate methods are needed to assess proteasome inhibitor specificity and efficacy in patients.

Purpose of the Study:

  • To develop and validate sensitive fluorescent reporters for profiling proteasome activity in living cells.
  • To compare the specificity and utility of different fluorescent probes for proteasome activity measurement.

Main Methods:

  • Synthesis and biochemical validation of novel fluorescent proteasome activity reporters.
  • Application of probes in gel-based assays, confocal microscopy, and flow cytometry.
  • Profiling proteasome activity in various mouse tissues, controlling for postlysis artifacts.

Main Results:

  • Seven synthesized probes successfully labeled proteasomes in cell lysates, with specificity influenced by the fluorescent dye.
  • Two differentially labeled probes demonstrated suitability for live-cell proteasome activity analysis.
  • Proteasome subunit activity was found to be regulated in an organ-specific manner in mouse tissues.

Conclusions:

  • Developed fluorescent reporters offer a sensitive and versatile tool for studying proteasome activity in living cells.
  • These methods can be applied to assess proteasome inhibitor pharmacodynamics in vivo.
  • Organ-specific regulation of proteasome activity was elucidated using these novel reporters.