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Updated: Feb 10, 2026
An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
Published on: July 15, 2015
[Effect of curcumin on expression of survivin, Bcl-2 and Bax in human multiple myeloma cell line]
Bo Liu1, Qing-Xian Bai, Xie-Qun Chen
1Department of Hematology, Xijing Hospital, The Fourth Medical University, Xi'an 210032, China.
Abstract:
To explore the mechanisms of suppression growth and induction apoptosis of curcumin on human multiple myeloma cell line RPMI8226, the suppressive effect of curcumin on RPMI8226 was examined by MTT assay; the induction apoptosis and cell cycle arrest of curcumin on RPMI8226 were determined by flow cytometry (FCM); the changes of survivin, Bcl-2, Bax mRNA levels were detected by RT-PCR. The results showed that curcumin obviously suppressed the proliferation of RPMI8226 in both time- and dose-dependent manners, and the IC(50) were 12.15 micromol/L, 4.9 micromol/L for 24 and 48 hours respectively. FCM indicated that the apoptosis ratio rose from 10.6% of untreated cells up to 36.9% of treated cells (p < 0.05), and curcumin arrested cell cycle of RPMI8226 at G(2)/M phase. RT-PCR showed that RPMI8226 cells expressed survivin, Bcl-2 strongly and Bax slightly; while RPMI8226 cells were treated with curcumin 10 micromol/L for 24 hours, the expressions of survivin, Bcl-2 mRNA were apparently down-regulated, and the expression of Bax mRNA was markedly up-regulated. It is concluded that curcumin can suppress the proliferation of human multiple myeloma cell line RPMI8226, and induce their apoptosis. The mechanism of antitumous effect of curcumin may be related to down-regulation of survivin, Bcl-2 mRNA and up-regulation of Bax mRNA.
Insights
Curcumin suppresses human multiple myeloma cell growth and induces apoptosis. Its anti-cancer mechanism involves regulating survivin, Bcl-2, and Bax mRNA levels.
Area of Science:
- Biochemistry
- Molecular Biology
- Cancer Research
Background:
- Human multiple myeloma is a hematological malignancy.
- Curcumin, a compound from turmeric, exhibits potential anti-cancer properties.
- Understanding curcumin's effects on myeloma cells is crucial for therapeutic development.
Purpose of the Study:
- To investigate the mechanisms by which curcumin suppresses growth and induces apoptosis in the human multiple myeloma cell line RPMI8226.
- To analyze the impact of curcumin on cell cycle progression and specific gene expressions related to apoptosis.
Main Methods:
- MTT assay was used to assess curcumin's suppressive effect on RPMI8226 cell proliferation.
- Flow cytometry (FCM) determined the induction of apoptosis and cell cycle arrest.
- RT-PCR analyzed changes in survivin, Bcl-2, and Bax mRNA levels.
Main Results:
- Curcumin significantly suppressed RPMI8226 proliferation in a time- and dose-dependent manner (IC50 values provided).
- FCM revealed a significant increase in apoptosis ratio (from 10.6% to 36.9%) and G2/M phase cell cycle arrest.
- RT-PCR showed curcumin down-regulated survivin and Bcl-2 mRNA while up-regulating Bax mRNA expression.
Conclusions:
- Curcumin effectively suppresses proliferation and induces apoptosis in human multiple myeloma RPMI8226 cells.
- The anti-tumor mechanism of curcumin is potentially linked to the modulation of survivin, Bcl-2, and Bax mRNA expression.
- These findings suggest curcumin as a potential therapeutic agent for multiple myeloma.
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