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Characterization of Aquatic Biofilms with Flow Cytometry
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Characterization of Aquatic Biofilms with Flow Cytometry

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Cell biologists sort things out: Analysis and purification of intracellular organelles by flow cytometry

G Böck1, P Steinlein, L A Huber

  • 1Institute for General and Experimental Pathology, University of Innsbruck, Fritz-Pregel Strasse 3, A-6020, Austria.

Trends in Cell Biology
|August 22, 2007
PubMed

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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