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Isolation and partial sequence of goat spleen prothymosin alpha
S Frillingos1, M Frangou-Lazaridis, K Seferiadis
1Laboratory of Biological Chemistry, University of Ioannina Medical School, Greece.
Molecular and Cellular Biochemistry
|November 13, 1991
Summary
Goat prothymosin alpha, a protein found in goats, shows high similarity to bovine and human versions. This acidic polypeptide is more concentrated in the spleen than the thymus in goats.
Area of Science:
- Biochemistry
- Immunology
- Molecular Biology
Background:
- Prothymosin alpha is an acidic protein involved in cellular processes.
- Its sequence and distribution vary across species.
Purpose of the Study:
- To isolate and characterize goat prothymosin alpha.
- To compare its sequence and tissue distribution with other species.
Main Methods:
- Isolation of goat prothymosin alpha from lymphoid and non-lymphoid tissues.
- Automatic sequencing of tryptic peptide fragments.
Main Results:
- Goat prothymosin alpha (109 amino acids, pI 3.5) was isolated.
- Higher concentration found in spleen compared to thymus.
- Closely resembles bovine (1 substitution) and human (3 substitutions) prothymosin alpha.
- Differs more significantly from rat and murine forms (2 deletions, 3 substitutions).
Conclusions:
- Goat prothymosin alpha exhibits high conservation across mammalian species.
- Tissue-specific expression patterns may differ, with higher splenic concentration in goats.