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In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
Published on: January 22, 2020
CD101 surface expression discriminates potency among murine FoxP3+ regulatory T cells
Irina Fernandez1, Robert Zeiser, Holger Karsunky
1Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela [corrected]
Journal of Immunology (Baltimore, Md. : 1950)
|August 22, 2007
Summary
CD101 expression identifies potent regulatory T cells (Treg) in mice. High CD101 expression on Treg correlates with enhanced suppression of immune responses and improved outcomes in graft-vs-host disease models.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD4+CD25+FoxP3+ regulatory T cells (Treg) show protective effects in autoimmunity and graft-vs-host disease (GvHD).
- Functional heterogeneity within Treg populations necessitates markers for identifying highly active cells.
Purpose of the Study:
- To identify surface markers that distinguish functionally active murine Treg.
- To investigate the role of CD101 in Treg function and GvHD.
Main Methods:
- Generated a rabbit monoclonal antibody against murine CD101.
- Analyzed CD101 expression on T cells using flow cytometry.
- Assessed Treg suppressive function in vitro and in a GvHD mouse model using bioluminescence imaging.
Main Results:
- CD101 was detected on a subset of CD4+CD25+ Treg and conventional memory T cells.
- CD101(high) Treg exhibited superior in vitro suppression compared to CD101(low) Treg.
- In vivo, CD101(high) Treg treatment reduced donor T cell expansion, improved survival, and decreased inflammation in a GvHD model.
Conclusions:
- CD101 expression is a reliable marker for identifying murine Treg with potent suppressor activity.
- CD101(high) Treg, particularly the CD62L(high) subpopulation, are effective in controlling GvHD.
