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Related Experiment Video

Updated: Jul 12, 2026

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

A gammaherpesviral internal repeat contributes to latency amplification.

Nagendra N Thakur¹, Susanne El-Gogo, Beatrix Steer

¹Institute of Molecular Immunology, Clinical Cooperation Group Hematopoietic Cell Transplantation, GSF National Research Center for Environment and Health, Munich, Germany.

|August 22, 2007

Summary

The 40 bp repeat in murine gammaherpesvirus 68 (MHV-68) is crucial for latency amplification and viral gene regulation. Its absence reduces splenomegaly and affects latency-associated gene expression during infection.

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Measurement of γHV68 Infection in Mice

Measurement of γHV68 Infection in Mice

Published on: November 22, 2011

Area of Science:

Virology
Immunology
Molecular Biology

Background:

Gammaherpesviruses cause significant human infections, especially in immunocompromised individuals.
The role of internal repeats in gammaherpesvirus pathogenesis is not well understood.

Purpose of the Study:

To investigate the biological role of the 40 bp internal repeat in murine gammaherpesvirus 68 (MHV-68).
To determine if the repeat functions independently or as part of open reading frame (ORF) M6.

Main Methods:

Construction of MHV-68 mutants lacking or with disrupted 40 bp repeat.
In vitro and in vivo infection models using laboratory mice.
Analysis of viral replication, splenomegaly, and latency-associated gene expression.

Main Results:

Loss of the 40 bp repeat did not significantly impact lytic replication.
Splenomegaly and the number of reactivating splenocytes were reduced in repeat mutants.
Disruption of ORF M6 did not affect lytic or latent infection.
Absence of the repeat reduced expression of latency-associated genes K3 and ORF72 in latently infected cells.

Conclusions:

The 40 bp repeat contributes to latency amplification in MHV-68.
The repeat may play a role in regulating viral gene expression during latency.