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Updated: Jul 12, 2026

Measurement of Cytosolic Ca2+ in Isolated Contractile Lymphatics
Published on: December 8, 2011
Length-dependence of lymphatic phasic contractile activity under isometric and isobaric conditions
Rongzhen Zhang1, Anatoliy A Gashev, David C Zawieja
1Department of Systems Biology & Translational Medicine, Texas A&M Health Science Center, College Station, Texas, USA.
Objective:
Experiments were designed to determine if the effect of preload on the spontaneous contractile activity of rat mesenteric lymphatics would be different under isometric and isobaric conditions.
Methods:
Isobaric protocols on cannulated, pressurized rat mesenteric lymphatics ( approximately 120 microm, ID) measured the effects of lumenal pressure on contraction amplitude, frequency, and rate of diameter change (dD/dt). Analogous protocols were conducted using a wire myograph to determine the effects of passive force (preload) on the characteristics of spontaneous, isometric force transients. Servo-control systems allowed responses to ramp or step increases in preload/pressure to be tested.
Results:
Under isobaric conditions, contraction amplitude was maximal at the lowest pressure tested (1 cm H(2)O), and progressively declined with pressure elevation to 10 cm H(2)O. Changes in dD/dt followed a similar pattern. In contrast, contraction frequency progressively increased with pressure. Under isometric conditions, the amplitude of spontaneous force transients was maximal at preloads of 0.3-0.4 mN, with dF/dt following a similar pattern; the average peak amplitude was approximately 15% of the maximal active contractile force developed during agonist stimulation. The frequency of the transients increased with preload from 0.05 to 0.3 mN, and remained constant at higher preloads. The amplitude-frequency product, an estimate of lymphatic pumping ability, reached a maximum value at 0.4 mN in isometric vessels and at 6 cm H(2)O in isobaric vessels.
Conclusions:
Isometric and isobaric methods yielded qualitatively similar indices of spontaneous contractile activity. However, the ranges of amplitude and frequency changes were much greater under isobaric conditions (3- to 5-fold) than under isometric conditions (50-80%).
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