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Related Experiment Videos

Differential phase-contrast BioCD biosensor.

Ming Zhao1, Wonryeon Cho, Fred Regnier

  • 1Department of Physics, Purdue University, West Lafayette, Indiana 47907-2036, USA. zhaom@purdue.edu

Applied Optics
|August 23, 2007
PubMed
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Common-path differential phase-contrast interferometry on a BioCD detects molecular binding. This technique achieves high mass sensitivity for antibody IgG detection, crucial for sensitive immunoassays.

Area of Science:

  • Bio-optical sensing
  • Interferometry
  • Surface science

Background:

  • Molecular binding detection is crucial for diagnostics.
  • Existing methods may lack sensitivity or specificity.
  • Bio-optical compact disks (BioCDs) offer a novel sensing platform.

Purpose of the Study:

  • To develop and validate a common-path differential phase-contrast interferometry method for BioCDs.
  • To assess the sensitivity and specificity of this technique for antibody detection.
  • To evaluate its performance in an immunoassay context.

Main Methods:

  • Utilized common-path differential phase-contrast interferometry.
  • Deposited antibody IgG proteins on BioCDs using lithography.
  • Employed spatial carrier-wave sideband demodulation for signal extraction.

Related Experiment Videos

  • Performed interferometric profilometry for surface height and mass sensitivity measurements.

    • Main Results:

    • Achieved surface height sensitivity down to 20 pm.
    • Demonstrated a mass sensitivity limit of 1.5 pg/mm, improving to 7 pg/mm in immunoassay conditions.
    • An IgG immunoassay showed low false positive (0.2%) and false negative (0.2%) rates.

    Conclusions:

    • Common-path differential phase-contrast interferometry is a highly sensitive method for detecting molecular binding on BioCDs.
    • The technique shows promise for robust immunoassays with minimal errors.
    • This approach enables precise measurement of surface dipole density changes.