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Updated: Jul 12, 2026
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
Immune response to ovalbumin following bisphenol A administration in mice fed with a low level of dietary protein
Mohammad Alizadeh1, Fusao Ota, Afework Kassu
1Department of Preventive Environment and Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima, Japan.
Background And Purpose:
We have previously shown that bisphenol A (BPA) augments T-helper (Th) 1 activity with no significant effects on an established oral tolerance to ovalbumin (OVA) in mice fed with a normal protein diet. The present study aimed to examine the effect of BPA on the immune response in a mouse model maintained on a very low protein diet (5% casein).
Methods:
Mice were fed on a 5% protein diet, together with either OVA (OVA-fed) or water (water-fed), immunized intraperitoneally with OVA at 3-week intervals and administered BPA between the 2 immunizations. A week after the last immunization, animals were sacrificed and examined by enzyme-linked immunosorbent assay for serum titers of total immunoglobulin E (IgE), OVA-specific IgE, immunoglobulin G (IgG), IgG1, IgG2a, and the production of interferon-gamma, interleukin (IL)-4, and IL-12.
Results:
In both BPA-treated and non-treated animals, OVA feeding resulted in lower titers of total and OVA-specific IgE, and OVA-specific IgG (p<0.05). There were higher levels of interferon-gamma (p<0.05), IL-4, and IL-12 (p<0.05) in animals with OVA tolerance following BPA treatment. However, IL-12 production was augmented only in BPA-treated water-fed animals (p<0.01).
Conclusion:
BPA administration in mice fed with a low level of dietary protein augmented Th1 cytokines more profoundly in the animals with OVA tolerance than in the non-tolerant animals.
