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17beta-estradiol stimulates proliferation of spermatogonia in experimental cryptorchid mice
En-Zhong Li1, De-Xue Li, Shi-Qing Zhang
1Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
Aim:
To investigate whether estrogen stimulates the proliferation of spermatogonia or induces spermatogenesis in cryptorchid mice.
Methods:
Mice were surgically rendered cryptorchid, then treated with different doses of 17beta-estradiol (E2) s.c. once a day. Mice were killed at sexual maturity (45 days of age), and histological analysis and immunofluorescence were performed. Serum follicle stimulating hormone (FSH), estradiol, testosterone and luteinizing hormone (LH) were measured.
Results:
Low doses of E2 had no notable effect on spermatogonia, but at higher doses, E2 stimulated the proliferation of spermatogonia.
Conclusion:
E2 has a dose-related mitogenic effect on spermatogonia.
