Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Active membranes studied by X-ray scattering.

A Giahi1, M El Alaoui Faris, P Bassereau

  • 1Institut für Röntgenphysik, Friedrich-Hund-Platz 1, 37073, Göttingen, Germany. agiahi@gwdg.de

The European Physical Journal. E, Soft Matter
|August 23, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Palmitoylated caveolin-1 enables endosome sorting of complex sphingolipids.

bioRxiv : the preprint server for biology·2025
Same author

Pump-probe X-ray holographic imaging of laser-induced cavitation bubbles with femtosecond FEL pulses.

Nature communications·2021
Same author

MICAL-L1 is required for cargo protein delivery to the cell surface.

Biology open·2021
Same author

Iterative micro-tomography of biopsy samples from truncated projections with quantitative gray values.

Physics in medicine and biology·2021
Same author

SARS E protein in phospholipid bilayers: an anomalous X-ray reflectivity study.

Physica. B, Condensed matter·2020
Same author

Spectral µCT with an energy resolving and interpolating pixel detector.

Optics express·2020

This study investigated active membranes using bacteriorhodopsin (BR) and X-ray scattering. Researchers found no changes in membrane spacing or fluctuations under varying light conditions, challenging active membrane theories.

Area of Science:

  • Biophysics
  • Membrane Biophysics
  • Structural Biology

Background:

  • Recent theories propose "active" membranes, where embedded proteins can drive systems out of equilibrium.
  • Bacteriorhodopsin (BR), a light-active transmembrane protein, is a model system to study these active membrane dynamics.
  • Understanding how active components influence membrane collective properties is crucial for biophysical research.

Purpose of the Study:

  • To investigate the effect of illumination on multilamellar phospholipid membrane stacks containing bacteriorhodopsin (BR).
  • To determine if the light-driven activity of BR alters the collective fluctuation properties of the membranes.
  • To experimentally test predictions of active membrane theories using X-ray scattering techniques.

Main Methods:

Related Experiment Videos

  • Utilized X-ray reflectivity, non-specular (diffuse) scattering, and grazing incidence scattering.
  • Studied multilamellar phospholipid membrane stacks reconstituted with bacteriorhodopsin (BR).
  • Controlled and varied illumination conditions applied to the membrane samples.

    • Main Results:

    • No detectable changes were observed in X-ray scattering curves under different illumination conditions.
    • The intermembrane spacing (d) remained constant, irrespective of light exposure.
    • Observed absence of non-equilibrium effects within the experimental parameters and detection limits.

    Conclusions:

    • The study found no evidence supporting theories predicting changes in collective membrane fluctuation properties due to active proteins like BR.
    • Experimental results indicate that BR, under the studied conditions, does not significantly alter the structural dynamics of the phospholipid membrane stacks.
    • The findings suggest limitations or specific conditions under which active membrane theories may apply.