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Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

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Related Experiment Video

Updated: Jul 12, 2026

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

Pattern of Wnt ligand expression during chick eye development.

E Rossi¹, F Siwiec, C Y I Yan

¹Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas

|August 24, 2007

Summary

This summary is machine-generated.

Wnt-5a is expressed in the dorsal region of developing chick eyes, specifically the retinal pigmented epithelium. This finding suggests Wnt-5a may serve as a marker for eye dorsoventral axis development.

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Last Updated: Jul 12, 2026

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Published on: January 17, 2016

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Published on: October 16, 2016

Area of Science:

Developmental biology
Ophthalmology
Molecular biology

Background:

The eye's dorsoventral axis is established before optic cup formation.
Signaling pathways like BMP4, Shh, and retinoic acid are crucial for maintaining this axis.
The role of Wnt ligands in optic axis development requires further investigation.

Purpose of the Study:

To investigate the potential role of Wnt ligands in establishing or maintaining the optic axis.
To analyze the expression patterns of Wnt ligands during early chick optic development.

Main Methods:

In situ hybridization was used to examine Wnt gene expression (Wnt-1, Wnt-3a, Wnt-4, Wnt-5a).
Analysis focused on the optic region during optic vesicle, early optic cup, and established optic cup stages.
Cryosections were utilized to confirm expression localization within the optic region.

Main Results:

Wnt-5a, but not Wnt-1, Wnt-3a, or Wnt-4, showed expression in the dorsal region of the developing eye starting at Hamburger and Hamilton stage 14 (HH14).
Cryosections confirmed Wnt-5a expression exclusively in the dorsal retinal pigmented epithelium.
Expression was observed from HH14 to HH19 in chick embryos.

Conclusions:

Wnt-5a is specifically expressed in the dorsal retinal pigmented epithelium of developing chick eyes.
Wnt-5a may function as a marker for the dorsal retinal pigmented epithelium in chick embryos.
These findings contribute to understanding the molecular mechanisms governing eye axis determination.