Related Experiment Video
Updated: Jul 12, 2026
09:15
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Published on: November 21, 2017
Temperature-dependent time-resolved fluorescence study of cinchonine alkaloid dication
Hirdyesh Mishra1, Sanjay Pant, Hera B Tripathi
1Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012 India. hirdyesh@mbu.iisc.ernet.in
Journal of Fluorescence
|August 24, 2007
Summary
Photoinduced excited state dynamics of cinchonine alkaloid dication (C(++)) reveal two distinct emitting species. The longer-lived species exhibits excited state solvent relaxation and charge transfer characteristics, influenced by chloride ion quenching.
Area of Science:
- Photochemistry
- Chemical Physics
- Spectroscopy
Background:
- Cinchonine alkaloid dication (C(++)) is a molecule of interest for studying excited state dynamics.
- Understanding photoinduced processes is crucial for various chemical and physical applications.
Purpose of the Study:
- To investigate the photoinduced excited state dynamical processes of cinchonine alkaloid dication (C(++)) over a wide temperature range.
- To elucidate the nature of different emitting species and their relaxation mechanisms.
Main Methods:
- Steady-state and nanosecond time-resolved fluorescence spectroscopy were employed.
- Temperature-dependent fluorescence studies were conducted to analyze dynamical processes.
Main Results:
- Two distinct emitting species with characteristic decay rates were identified in C(++).
- The longer-lived species demonstrated excited state solvent relaxation (tau(r) ≈ 6 ns) and charge transfer character.
- This longer-lived species showed increased sensitivity to chloride ion quenching and concentration quenching.
Conclusions:
- The study reveals complex excited state dynamics in C(++) involving solvent relaxation and potentially energy migration.
- The findings provide insights into the photophysical behavior of alkaloid dications.
