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Updated: Jul 12, 2026
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
Published on: January 21, 2020
An MTT-based method for quantification of periodontal ligament cell viability
1Department of Conservative Dentistry, Oral Science Research Centerm College of Dentistry, Yonsei University, Seoul, Korea.
Objective:
Survival of periodontal ligament cells is crucial issue after tooth replantation. To understand this matter, we introduced MTT assay, which can be used as a tool for measuring the viability of periodontal ligament cells from extracted rat molars.
Materials And Methods:
The maxillary molars of 4-week-old Sprague-Dawley white female rats were used. Ten teeth of each immediate, 1 h Viaspan and 1 h dry were processed for MTT evaluation. Another 10 teeth from each group were treated in the same manner as above, but were replanted into their original socket. After 2 weeks, the animals were killed and the prevalence of resorption pits was evaluated.
Results:
In vivo MTT assay corresponded with the histological results of the resorption pits (P
Conclusions:
In vivo MTT measurements were consistent with the histologic observations and suggest that the in vivo MTT assay could be a tool for evaluating the viability of periodontal ligament cells directly from the extracted root surface. The advantages are shorter analysis time compared with animal or cell culture experiments, easy manipulation, clear quantification and immediate identification of the vital cells.
