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Related Experiment Videos

Transcriptomal profiling of site-specific Ras signals.

Lorena Agudo-Ibáñez1, Fátima Núñez, Fernando Calvo

  • 1Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas (CSIC), Departamento de Biología Molecular, Unidad de Biomedicina, CSIC-Universidad de Cantabria, Santander, Spain.

Cellular Signalling
|August 24, 2007
PubMed
Summary
This summary is machine-generated.

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Ras proteins in different cell locations activate distinct gene programs. While many targets overlap, specific signals from plasma membranes and endomembranes uniquely regulate gene expression, impacting cellular transformation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Ras proteins are key regulators of cellular signaling pathways.
  • Ras proteins localize to distinct cellular compartments, including plasma-membrane microdomains and endomembranes.
  • The functional consequences of Ras localization on transcriptional programs are not fully understood.

Purpose of the Study:

  • To investigate the impact of Ras protein localization on gene expression.
  • To identify distinct transcriptional targets of Ras signals from specific cellular microenvironments.
  • To understand how site-specific Ras activation influences cellular transformation.

Main Methods:

  • Utilized microarray technology to analyze gene expression profiles.
  • Engineered NIH3T3 cells to express H-RasV12 selectively tethered to distinct cellular locations.

Related Experiment Videos

  • Compared transcriptomes resulting from site-specific Ras activation.

    • Main Results:

    • Site-specific Ras activation resulted in transcriptomes with significant overlap but also distinct genetic signatures.
    • Identified 121 genes uniquely regulated by Ras signals from plasma-membrane microdomains.
    • Found 9 genes exclusively regulated by Ras signals from endomembranes.
    • Identified 31 common genes regulated by site-specific Ras signals that induce cellular transformation, including those for Vitamin D receptor and p120-GAP.

    Conclusions:

    • Ras protein localization significantly influences the resulting gene expression programs.
    • Distinct cellular compartments orchestrate unique transcriptional responses to Ras signaling.
    • Understanding these site-specific Ras signals is crucial for comprehending Ras-induced cellular transformation.