Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Variance in multiplex suspension array assays: microsphere size variation impact.

Brian P Hanley1, Li Xing, R Holland Cheng

  • 1Microbiology Graduate Group, University of California, Davis, CA 95616, USA. bphanley@ucdavis.edu

Theoretical Biology & Medical Modelling
|August 25, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety, Pharmacokinetics, and Pharmacodynamics of SHR6508, a Calcium-Sensing Receptor Agonist, in Maintenance Hemodialysis Patients With Secondary Hyperparathyroidism: A Multicenter, Randomized, Double-Blind, Placebo-Controlled Phase 1 Study.

Kidney medicine·2026
Same author

Mitochondrial-derived peptide MOTS-c activates metabolic signaling but blunts reparative function in human mesenchymal stromal cells.

Inflammation and regeneration·2026
Same author

Laparoscopic proximal gastrectomy with esophagogastrostomy using overlap method combined with gastric remnant "U"-shaped fold: a retrospective cohort study.

Frontiers in surgery·2026
Same author

Laser polishing for integrated enhancement of thin-walled LPBFed dental Co-Cr-Mo alloy frameworks.

Journal of the mechanical behavior of biomedical materials·2026
Same author

Association between CT-evaluated Poststenotic Dilatation in Human Renal Artery Stenosis and Kidney Release of MCP-1.

Radiology·2026
Same author

<i>Lyar</i> contributes to cell cycle progression and multi-lineage differentiation in mouse embryonic stem cells.

Frontiers in genetics·2026
Same journal

The impact of natural disasters on the spread of COVID-19: a geospatial, agent-based epidemiology model.

Theoretical biology & medical modelling·2024
Same journal

Assessing countermeasures during a hepatitis A virus outbreak among men who have sex with men.

Theoretical biology & medical modelling·2021
Same journal

The effect of men who have sex with men (MSM) on the spread of sexually transmitted infections.

Theoretical biology & medical modelling·2021
Same journal

Analysis of international traveler mobility patterns in Tokyo to identify geographic foci of dengue fever risk.

Theoretical biology & medical modelling·2021
Same journal

Markov modelling of viral load adjusting for CD4 orthogonal variable and multivariate conditional autoregressive mapping of the HIV immunological outcomes among ART patients in Zimbabwe.

Theoretical biology & medical modelling·2021
Same journal

On the relationship between inhibition and receptor occupancy by nondepolarizing neuromuscular blocking drugs.

Theoretical biology & medical modelling·2021
See all related articles

Microsphere size variation significantly impacts Luminex assay results. Optimizing manufacturing processes and providing detailed size metrics can improve assay precision and reliability.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Materials Science

Background:

  • Luminex suspension microarray assays are widely utilized in various scientific applications.
  • Assay readings generated by this technology exhibit significant variability, impacting data reliability.

Purpose of the Study:

  • To investigate the contribution of microsphere size variation to the overall variance observed in Luminex suspension microarray assays.
  • To identify potential strategies for mitigating assay variability through manufacturing process adjustments.

Main Methods:

  • Transmission electron microscopy was employed to visualize and measure microsphere size.
  • Statistical analysis was performed to correlate microsphere size distribution with assay signal distribution.
  • The contribution of microsphere size variation to assay variance was quantified.

Related Experiment Videos

Main Results:

  • Microsphere size variations were observed in distinct, stepwise increments.
  • A strong correlation was established between microsphere diameter distribution and the distribution of fluorescent events in assays.
  • The study estimated the impact of microsphere size variation on overall assay variance.

Conclusions:

  • Microsphere diameter variation is a probable significant cause of variance in suspended microsphere assay results.
  • Adjustments in the manufacturing process, focusing on tighter size control, can potentially reduce assay variability.
  • Recommended metrics for users include mean size, median size, skew, sigma multiple, and standard deviation to better interpret assay data.