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Updated: Jul 12, 2026
Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
Published on: May 16, 2020
In vitro differentiation of murine embryonic stem cells into keratinocyte-like cells
Ingo Haase1, Renate Knaup, Maria Wartenberg
1Department of Dermatology, University of Cologne and Center for Molecular Medicine, University of Cologne (CMMC), Joseph-Stelzmann-Strasse 9, D-50924 Cologne, Germany. Ingo.Haase@uni-koeln.de
Abstract:
Embryonic stem (ES) cells are omnipotent; they can differentiate into every cell type of the body. The development of culture conditions that allow their differentiation has made it conceivable to produce large numbers of cells with lineage-specific characteristics in vitro. Here, we describe a method by which murine ES cells can be differentiated into cells with characteristics of epidermal keratinocytes. Keratinocyte-like cells were isolated from embryoid bodies and grown in culture. Potential applications of this method are the in vitro differentiation of cells of interest from ES cells of mice with lethal phenotypes during embryonic development and the production of genetically modified epidermal keratinocytes that could be used as temporary wound dressing or as carriers of genes of interest in gene therapeutic treatments.
