Related Experiment Video
Updated: Jul 12, 2026

Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
Published on: September 29, 2016
Sulfur dioxide derivatives increase a hyperpolarization-activated inward current in dorsal root ganglion neurons
Zhengqing Du1, Yufen Zhou, Pin Yang
1Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, PR China. duzhengqing@sxu.edu.cn
Abstract:
The effect of derivatives of sulfur dioxide (SO(2)), a common air pollutant, which exists in vivo at equilibrium between bisulfate and sulfite, was studied on hyperpolarization-activated cation current (I(h)) in cultured post-natal dorsal root ganglion (DRG) neurons using the whole cell configuration of patch-clamp technique. SO(2) derivatives increased I(h) current in a dose and voltage-dependent manner. The EC(50) value was 25 microM and the Hill coefficient was 1.44. 50 microM SO(2) derivatives significantly shifted the activation curve of I(h) in the hyperpolarizing direction by 5.5 mV. The reversal potential of I(h) was shifted to 5.2 mV in positive direction by 10 microM SO(2) derivatives. According to the functional role of I(h), the increase of I(h) should result in an enhanced neuronal excitability, which was possibly the basis for neuropathic pain.
More Related Videos
09:34Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure
Published on: August 27, 2019
06:34In Vivo Thoracic Dorsal Root Ganglia (DRG) Calcium Imaging and ECG Recording for Studying Peripheral Nerve Stimulation
Published on: August 16, 2024
Related Concept Videos
The Role of Ion Channels in Neuronal Computation
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
Integration of Synaptic Events
Ligand-Gated Ion Channel Receptor: Gating Mechanism
Excitatory and Inhibitory Effects of Neurotransmitters
Action Potentials
Local Anesthetics: Differential Sensitivity of Nerve Fibers