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Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

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Published on: March 25, 2016

Tetraspanin CD151 promotes cell migration by regulating integrin trafficking.

Li Liu¹, Bo He, Wei M Liu

¹Vascular Biology Center, Cancer Institute, Department of Medicine and Department of Molecular Science, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA.

The Journal of Biological Chemistry

|August 25, 2007

Summary

Tetraspanin CD151 regulates cell migration by controlling integrin trafficking. Its internalization via a YXXφ motif is crucial for cell motility on extracellular matrices.

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Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Tetraspanin CD151 is known to associate with integrins.
The precise mechanism of CD151's regulation of integrin-dependent cell migration remains unclear.
CD151 is found in both the plasma membrane and intracellular vesicles.

Purpose of the Study:

To elucidate the mechanism by which CD151 regulates integrin-dependent cell migration.
To investigate the role of CD151 trafficking in cell motility.

Main Methods:

Investigated the co-localization and trafficking of CD151 and associated integrins (α3β1, α5β1, α6β1) using endocytosis studies.
Utilized site-directed mutagenesis to analyze the function of the YRSL endocytosis/sorting motif in CD151.
Assessed the impact of CD151 internalization on cell migration on laminin and integrin internalization.

Main Results:

CD151 and its associated integrins co-internalize and accumulate in intracellular vesicles.
Mutation of the YRSL motif in CD151 significantly reduced its internalization.
Disruption of CD151 trafficking abolished CD151-promoted cell migration and reduced integrin internalization.

Conclusions:

CD151 internalization, mediated by its YXXφ motif, is essential for promoting integrin-dependent cell migration.
Integrin trafficking, modulated by CD151 endocytosis, plays a critical role in regulating cell motility.