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Updated: Jul 12, 2026
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
Published on: March 10, 2020
[Cloning and expression of PAL gene of Legionella pneumophila]
Yu Tian1, Jian-ping Chen, Chun-lei Yang
1Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To develop DNA vaccine for Legionella pneumophila.
Methods:
PAL gene of Legionella pneumophila was amplified with PCR. The amplified DNA was ligated to pcDNA3.1(+) vector. The recombinant plasmid, which was identified by restriction analysis, PCR and sequence analysis, was named pcDNA3.1-PAL. The NIH3T3 cells were transfected with the recombinant plasmid pcDNA3.1-PAL by Lipofection. Transient and stable expression products of the PAL gene were detected by immunofluorescence and RT-PCR.
Results:
The recombinant plasmid pcDNA3.1-PAL expressed PAL protein in the eukaryotic cell NIH3T3.
Conclusion:
This study has built a foundation for the development of PAL gene DNA vaccine for Legionella pneumophila.
