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Updated: Jul 12, 2026
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Published on: May 4, 2018
[Effects of hypotonic solution on the proliferation, alkaline phosphatase activity and [Ca2+]i concentration of
Wei-qun Zhang1, Zhi-min Zhu, Yong-lie Chao
1Department of Prosthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the effects of static-stretch from the hypotonic solution on the proliferation, alkaline phosphatase activity and [Ca2+]i in the osteoblast-like cells.
Methods:
Mechanical loading was introduced by swelling in the hypotonic solution. In vitro cultured MG63 were incubated under continuous swelling of 277, 240 and 163mOsm for 2h, 4h, 8h, 12h, 24h and 48h. The cell proliferation was detected by MTT assay. ALPase and [Ca2+]i were determined by modified enzyme dynamic method and OCPC respectively.
Results:
The cell proliferation, ALP activity and [Ca2+]i increased slowly under continuous static-stretch of 277 and 240mOsm. The cell proliferation was inhibited under 163mOsm, with a sharp increase of [Ca2+]i at 8h (11.383 +/- 0.111) and an increase of ALPase activity (0.326 +/- 0.002).
Conclusion:
The static-stretch induced from the hypotonic solution has an impact on the proliferation, differentiation, ALPase and Ca2+-ATPase of the MG63. The [Ca2+]i is correlated with the ALPase.
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