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Updated: Jul 12, 2026
Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
[Purification of a novel chitosanase from Metarhizium guizhouense in solid-state fermentation with affinity
Li Yang1, Tao Zhang, Xiao-hong Li
1Department of Biopharmaceutics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Objectives:
To find a new source of chitosanase from Metarhizium guizhouense in solid state fermentation through purification techniques with affinity chromatography and molecular sieving.
Methods:
Cross-linked chitosan resins adsorbed chitosanase from crude enzyme, which was then eluted with 5% per hundred Cu2+. The elute was dialyzed, concentrated and separated by Superdex75 chromatography.
Results:
Under the optimal conditions, the chitosanase activity reached a maximum of 83.09 +/- 2.9 U for one gram of dry medium after 120 hours of culture. The chitosanase was purified, with 43.52% enzyme activity maintained. The specific activity of the purified enzyme reached 106.39 U/mg. The chitosanase had a molecular mass of 50.3 x 10(3).
Conclusion:
A new source of chitosanase from Metarhizium guizhouense under solid state fermentation conditions is obtained. Affinity chromatography and molecular sieving can be used to purify chitosanase.
