Related Experiment Video
Updated: Jul 12, 2026

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
Published on: May 4, 2018
The distribution patterns of bases of protein-coding genes, non-coding ORFs, and intergenic sequences in pseudomonas
1School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. fbguo@uestc.edu.cn
Abstract:
The distribution patterns of bases of DNA fragments in different regions in P. aeruginosa genome are analyzed in this paper. It's shown that 5565 protein-coding genes, 17315 non-coding ORFs, and 1104 intergenic sequences are located into seven clusters based on their base frequencies. Almost all the protein-coding genes are contained in one of the seven clusters. The significant difference of base frequencies among three codon positions in high GC genome, which arouse the division between the distribution patterns of bases of six reading frames of protein-coding genes, is responsible for the appearance of the clustering phenomenon. In the light of the clustering phenomenon, the author supposes that the anitisense strand ORFs, particularly those corresponding to Frame 2' and Frame 3', may not code for proteins in P. aeruginosa genome.
Related Concept Videos
Prokaryotic Gene Structure and Organization
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Gene Regulation in Microbial Communities: Quorum Sensing
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Genome Size and the Evolution of New Genes
Operon Model
