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Updated: Jul 12, 2026
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Quantification of angiogenesis in estrogen receptor-positive and negative breast carcinoma
Jb Parentes-Vieira1, Pv Lopes-Costa, Cg Pires
1Department of Gynecology, Federal University of Piauí, Teresina, Piauí, Brazil. beneditoborges@globo.com.
Background:
The objective of this study was to evaluate angiogenesis according to CD34 antigen expression in estrogen receptor (ER)-positive and negative breast carcinomas.
Methods:
This study comprised 64 cases of infiltrating ductal carcinoma in postmenopausal women divided into two groups: Group A: ER-positive, n = 35; and Group B: ER-negative, n = 29. The anti-CD34 monoclonal antibody was used as a marker for endothelial cells. Microvessel count was carried out in 10 fields per slide using a 40x objective lens (magnification 400x). Statistical analysis of the data was performed using Student's t-test (p < 0.05).
Results:
The mean number of vessels stained with the anti-CD34 antibody in the estrogen receptor-positive and negative tumors was 23.51 +/- 1.15 and 40.24 +/- 0.42, respectively. The number of microvessels was significantly greater in the estrogen receptor-negative tumors (p < 0.001).
Conclusion:
ER-negative tumors have significantly greater CD34 antigen expression compared to ER-positive tumors.
