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PARE: a tool for comparing protein abundance and mRNA expression data
Eric Z Yu1, Anne E Counterman Burba, Mark Gerstein
1Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT 06520, USA. acmg@bioinfo.mbb.yale.edu
BMC Bioinformatics
|August 28, 2007
Summary
A new web tool, PARE, correlates protein abundance and mRNA expression data. This tool helps researchers compare these two crucial biological quantities and identify outliers for further study.
Area of Science:
- Proteomics
- Genomics
- Bioinformatics
Background:
- Advancing techniques for measuring protein abundance are generating large datasets.
- Protein expression is linked to mRNA levels, but the relationship requires detailed analysis.
Purpose of the Study:
- To develop a tool for correlating protein abundance and mRNA expression.
- To facilitate comparative studies between protein and mRNA levels.
Main Methods:
- Development of the PARE (Protein Abundance and mRNA Expression) web tool.
- Global and subset-based correlation analysis of protein and mRNA data.
- Identification of correlation outliers.
Main Results:
- The PARE web tool enables direct comparison of protein and mRNA quantities.
- Users can analyze specific protein subsets based on function or complexes.
- Outlier data points are highlighted for potential further investigation.
Conclusions:
- PARE is expected to aid the proteomics community in comparative mRNA and protein abundance studies.
- The tool facilitates deeper insights into the relationship between gene and protein expression levels.
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