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Updated: Jul 12, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
Published on: September 15, 2021
Evolutionary impact of limited splicing fidelity in mammalian genes
Chaolin Zhang1, Adrian R Krainer, Michael Q Zhang
1Cold Spring Harbor Laboratory, NY 11724, USA.
Abstract:
The functional significance of most alternative splicing (AS) events, especially frame-shifting ones, has been controversial. Using human-mouse comparison, we demonstrate that frame-preserving AS events adapt and get fixed more rapidly than frame-shifting AS events; selection for smaller exon size is stronger in frame-preserving exons than in frame-shifting ones. These results suggest AS events introducing mild changes are generally favored during evolution and explain the excess of shorter, frame-preserving cassette exons in present mammalian genomes.
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