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Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Catenins01:23

Catenins

Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

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Related Experiment Video

Updated: May 13, 2026

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

Cep97 and CP110 suppress a cilia assembly program.

Alexander Spektor1, William Y Tsang, David Khoo

  • 1Department of Pathology and NYU Cancer Institute, New York University School of Medicine, Smilow Research Center, 522 First Avenue, New York, NY 10016, USA.

Cell
|August 28, 2007
PubMed
Summary

Researchers identified Cep97, a protein that recruits CP110 to centrosomes. Loss of Cep97 or CP110 promotes primary cilia formation, suggesting they collaborate to inhibit ciliogenesis and impact human ciliary diseases.

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Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Centrosomes, crucial for cell division, also nucleate cilia assembly.
  • Key regulators controlling centriole fate (centrosome vs. cilia) are largely unknown.

Purpose of the Study:

  • To identify novel regulators of centrosome and cilia formation.
  • To elucidate the roles of CP110 and its interacting partners in these processes.

Main Methods:

  • Purification of protein complexes associated with CP110.
  • Depletion and dominant-negative mutant expression studies.
  • Analysis of centrosome, spindle, and primary cilia formation.

Main Results:

  • Identified Cep97 as a novel protein that recruits CP110 to centrosomes.
  • Cep97 depletion or inhibition causes CP110 loss, spindle defects, and polyploidy.
  • Loss of Cep97 or CP110 promotes primary cilia formation, while CP110 overexpression inhibits it.

Conclusions:

  • Cep97 and CP110 function together to suppress ciliogenesis in growing cells.
  • Understanding Cep97 and CP110 regulation is crucial for deciphering human ciliary diseases like those affecting kidneys and retinas.