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Related Experiment Videos

Interaction between lysozyme and poly(acrylic acid) microgels.

Christian Johansson1, Per Hansson, Martin Malmsten

  • 1Department of Pharmacy, Uppsala University, P.O. Box 580, SE-751 23 Uppsala, Sweden. christian.johansson@farmaci.uu.se

Journal of Colloid and Interface Science
|August 28, 2007
PubMed
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Lysozyme forms a charged shell within poly(acrylic acid) microgels, affecting their swelling and structure. This shell formation influences protein loading and particle behavior under varying conditions.

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Protein Interactions

Background:

  • Understanding protein-microgel interactions is crucial for drug delivery and biomaterial design.
  • Poly(acrylic acid) microgels are widely used due to their tunable properties.
  • Lysozyme is a model protein for studying protein-biomaterial interactions.

Purpose of the Study:

  • To investigate the interaction between lysozyme and poly(acrylic acid) microgels.
  • To elucidate the distribution and effect of lysozyme within microgels.
  • To study the influence of pH, ionic strength, and protein concentration on these interactions.

Main Methods:

  • Micromanipulator-assisted light microscopy
  • Confocal microscopy

Related Experiment Videos

  • Circular dichroism spectroscopy

    • Main Results:

    • Lysozyme forms a nonuniform shell within microgels under various conditions.
    • Shell formation increases lysozyme loading and induces microgel deswelling.
    • Shells exhibit a net positive charge and variable lysozyme exchange kinetics.

    Conclusions:

    • Lysozyme-microgel interactions lead to shell formation and altered microgel properties.
    • Ionic strength significantly impacts shell formation and protein exchange.
    • The formed shells can restrict diffusion, with pore sizes below 4 nm at slow exchange rates.