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Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

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Related Experiment Video

Updated: Jul 12, 2026

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Electrophoretic separation of large DNAs using steric confinement.

Jérôme Mathé1, Jean-Marc Di Meglio, Bernard Tinland

  • 1Matériaux et Polymères aux Interfaces, Université d'Evry-Val d'Essonne, 91025 Evry Cedex, France.

Journal of Colloid and Interface Science
|August 28, 2007
PubMed
Summary

This study introduces a novel method for separating large DNA molecules using steric confinement, observing distinct separation at small gaps and bulk behavior at larger ones.

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09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

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Published on: April 20, 2012

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
13:46

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Published on: September 29, 2011

Area of Science:

  • Biophysics
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Electrophoretic separation is crucial for analyzing large DNA molecules.
  • Traditional gel electrophoresis faces limitations with very large DNA fragments.

Purpose of the Study:

  • To explore an alternative DNA separation technique using steric confinement.
  • To investigate the relationship between confinement thickness and DNA electrophoretic mobility.

Main Methods:

  • Electrophoretic separation of large DNA was performed using steric confinement between solid walls.
  • Fluorescence video microscopy was employed to observe DNA mobility changes.
  • The effect of confinement thickness on separation was systematically studied.

Main Results:

  • DNA separation was achieved at small confinement thicknesses.
  • A transition to bulk behavior (no separation) occurred at approximately 4 micrometers confinement thickness.
  • Observed phenomena suggest confinement-induced changes in DNA conformation and mobility.

Conclusions:

  • Steric confinement offers a gel-free method for large DNA separation.
  • Confinement thickness critically influences electrophoretic mobility and separation efficiency.
  • Further research is needed to fully elucidate the mechanisms behind confinement-driven DNA behavior.