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Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
Published on: February 24, 2023
Identification of major immunogenic proteins of Mycoplasma synoviae isolates
Rebeka Lucijana Bercic1, Brigita Slavec, Miha Lavric
1Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, Domzale, Slovenia.
Abstract:
Mycoplasma synoviae isolates differ in patterns of immunogenic proteins, but most of them have not been identified yet. The main aim of this study was their identification in two closely related M. synoviae isolates, ULB 02/P4 and ULB 02/OV6, recovered recently from chickens in Slovenia. N-terminal sequencing identified 17 M. synoviae proteins. Amongst them were 14 major, highly expressed but previously unidentified proteins, including enzymes, chaperones and putative lipoproteins. ULB 02/P4 proteins with increasing molecular weight (M(w)) in the region above the lipoprotein MSPB (approximately 40 kDa) were elongation factor EF-Tu, enolase, NADH oxidase, haemagglutinin MSPA, ATP synthase beta chain, trigger factor, pyruvate kinase and chaperone DnaK. Enolase (approximately 47 kDa) seemed to be immunogenic for chickens infected with M. synoviae, whereas EF-Tu, which might cross-react with antibodies to the P1 adhesin of Mycoplasma pneumoniae, was not. ULB 02/OV6 synthesized several immunogenic proteins and those with M(w) of approximately 70, 78, 82, 90, 110 and 160 kDa, cross-reacted with antibodies to Mycoplasma gallisepticum. They remain to be identified, because besides putative lipoproteins, protein bands of 78, 82, 85 and 110 kDa contained also dehydrogenase PdhD, elongation factor EF-G, enzyme PtsG and putative neuraminidase, respectively.
Insights
This study identified 14 new immunogenic proteins in Mycoplasma synoviae isolates from chickens. Understanding these proteins aids in developing better diagnostics and vaccines for avian mycoplasmosis.
Area of Science:
- Veterinary Microbiology
- Avian Pathology
- Proteomics
Background:
- Mycoplasma synoviae (M. synoviae) causes significant economic losses in the poultry industry.
- The immunogenic proteins of M. synoviae are crucial for host immune response but many remain unidentified.
- Understanding these proteins is key to developing effective vaccines and diagnostic tools.
Purpose of the Study:
- To identify previously unidentified immunogenic proteins in two closely related M. synoviae isolates (ULB 02/P4 and ULB 02/OV6) from Slovenian chickens.
- To characterize the protein profiles of these isolates and assess their immunogenicity.
- To provide insights into the molecular basis of M. synoviae pathogenesis.
Main Methods:
- N-terminal sequencing of M. synoviae proteins.
- Analysis of protein profiles based on molecular weight (M(w)).
- Cross-reactivity testing with antibodies against other Mycoplasma species.
Main Results:
- Seventeen M. synoviae proteins were identified, including 14 major, highly expressed, and previously unknown proteins (enzymes, chaperones, putative lipoproteins).
- Proteins identified in isolate ULB 02/P4 included elongation factor EF-Tu, enolase, NADH oxidase, haemagglutinin MSPA, ATP synthase beta chain, trigger factor, pyruvate kinase, and chaperone DnaK.
- Isolate ULB 02/OV6 synthesized several immunogenic proteins that cross-reacted with antibodies to Mycoplasma gallisepticum, including dehydrogenase PdhD, elongation factor EF-G, enzyme PtsG, and putative neuraminidase.
Conclusions:
- The study successfully identified numerous novel immunogenic proteins in M. synoviae isolates.
- Enolase was identified as potentially immunogenic in M. synoviae-infected chickens.
- Further identification of proteins in ULB 02/OV6 is needed to fully understand its immunogenic profile and potential cross-reactivity with M. gallisepticum.
