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Updated: Jul 12, 2026
10:01
The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
Published on: September 27, 2016
Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination
Wei Jie Qin1, Lin Yue Lanry Yung
1Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
Nucleic Acids Research
|August 28, 2007
Summary
This study introduces a gold nanoparticle method for DNA detection and quantification. The assay accurately identifies single nucleotide polymorphisms (SNPs) and offers potential for simple, quantitative DNA analysis.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Biology
Background:
- Sequence-specific DNA detection is crucial for gene expression profiling, disease diagnosis, drug discovery, and forensics.
- Existing methods for DNA detection and single nucleotide polymorphism (SNP) discrimination can be complex or lack precise quantification.
Purpose of the Study:
- To develop a gold nanoparticle-based method for sensitive and quantitative DNA detection.
- To demonstrate the capability of this method for discriminating single nucleotide polymorphisms (SNPs).
Main Methods:
- Utilized gold nanoparticles to form defined nanoparticle-DNA conjugate groupings with target/linker DNA.
- Employed gel electrophoresis for characterization and quantification of these conjugate groupings.
- Investigated the discrimination of single base mismatches in DNA sequences.
Main Results:
- Established a linear correlation between the amount of target DNA and the formation of conjugate groupings.
- Achieved precise quantification of single-stranded DNA based on conjugate grouping formation.
- Demonstrated single base mismatch discrimination for both end- and center-base mismatches in SNP detection.
Conclusions:
- The gold nanoparticle method enables quantitative DNA detection and precise SNP discrimination.
- This approach offers a potentially simple and effective assay for DNA analysis.
- The findings may advance the development of new diagnostic and forensic tools.
Related Concept Videos
01:05Single Nucleotide Polymorphisms-SNPs
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
02:26Comparing Copy Number Variations and SNPs
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...