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Updated: Jul 12, 2026
Isolation and Identification of Limbal Niche Cells
Published on: October 27, 2023
[In vitro phenotypic characterization of human limbal epithelial cells]
Gabriel Andrés Luna-Baca1, Yonathan Garfias, Atzin Robles-Contreras
1Unidad de Investigación, Instituto de Oftalmología Fundación Conde de Valenciana, México DF, México.
Introduction:
Human limbal epithelial cells (huLEC) have been used for clinical purposes in ocular surface diseases to promote rapid re-epithelisation and restore corneal epithelium integrity. However, in Mexico this technique has not been fully developed. This study was conducted to characterize the huLEC phenotype expanded in vitro using a cell culture technique.
Material And Methods:
Cells were obtained from limbal tissue, cultured in KSFM medium and analyzed for the expression of vimentin, K, K19, p63, K12, by flow cytometry and immuno-fluorescence.
Results:
The phenotype of cultured cells was vimentin+K+K19+ p63+K12-.
Conclusions:
Our results suggest that under these culture conditions huLEC maintained their stem cell phenotype. This culture technique could be used for clinical purposes in Mexico.
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