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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
Multiplexed microsphere diagnostic tools in gene expression applications: factors and futures
Gwendolyn A Lawrie1, Jodie Robinson, Simon Corrie
1Centre for Nanotechnology and Biomaterials,The University of Queensland, St Lucia, Australia.
International Journal of Nanomedicine
|August 29, 2007
Summary
Microsphere-based assays offer advanced gene expression profiling but require standardized parameters for reliable quantification. This study identifies key parameters crucial for accurate gene expression data from these novel platforms.
Area of Science:
- Biotechnology
- Molecular Biology
- Genomics
Background:
- Microarrays have faced challenges regarding data integrity and standardization in gene expression profiling.
- Microsphere-based assays are emerging as a next-generation diagnostic tool for gene expression analysis.
Purpose of the Study:
- To identify critical parameters for controlling information derived from microsphere-based hybridizations for gene expression quantification.
- To initiate the establishment of a standard set of parameters for reporting data from microsphere assays.
Main Methods:
- Investigated microsphere-based assays for gene expression profiling.
- Focused on identifying parameters important for quantifying low abundant genes.
Main Results:
- Identified several key parameters that influence the quality of gene expression data from microsphere assays.
- Highlighted variability in these parameters across different studies.
Conclusions:
- Standardization of parameters is essential for reliable gene expression quantification using microsphere-based assays.
- Further research is needed to establish a comprehensive set of required parameters for publication.
