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Microfluidics in Assessing Platelet Function
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Microfluidics in Assessing Platelet Function

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Different methodologies for evaluating the effect of clopidogrel on platelet function in high-risk coronary artery

R Paniccia1, E Antonucci, A M Gori

  • 1Department of Medical and Surgical Critical Care, Thrombosis Centre, University of Florence, Florence, Itlay. rita.paniccia@unifi.it

Journal of Thrombosis and Haemostasis : JTH
|August 29, 2007
PubMed

Insights

The VerifyNow assay correlates well with light transmission aggregation for measuring residual platelet reactivity in coronary artery disease patients. However, the Platelet Function Analyzer-100 (PFA-100) assay did not show significant correlation, indicating a need for further clinical validation of point-of-care systems.

Area of Science:

  • Cardiology
  • Clinical Chemistry
  • Pharmacology

Background:

  • Point-of-care (POC) systems offer rapid assessment of residual platelet reactivity (RPR).
  • Coronary artery disease (CAD) patients on dual antiplatelet therapy require monitoring of platelet function.

Purpose of the Study:

  • Compare the Platelet Function Analyzer-100 (PFA-100) and VerifyNow P2Y12 assays against light transmission aggregation (LTA).
  • Evaluate the utility of these POC systems in assessing RPR in CAD patients.

Main Methods:

  • 1267 CAD patients undergoing percutaneous coronary intervention were studied.
  • PFA-100 closure times (CTs) and VerifyNow P2Y12 Assay results were compared with LTA induced by ADP (2 and 10 micromol L(-1)).
  • Vaso-dilator-stimulated phosphoprotein (VASP) phosphorylation assay was performed in a subgroup.

Main Results:

  • VerifyNow showed significant correlation and agreement with LTA (2 and 10 micromol L(-1) ADP).
  • PFA-100 C/ADP CT results did not correlate significantly with LTA.
  • VASP platelet reactivity index correlated significantly with both ADP-LTA and VerifyNow.

Conclusions:

  • The VerifyNow assay demonstrates good correlation with LTA for RPR assessment.
  • The PFA-100 C/ADP assay is not a reliable alternative to LTA in this patient group.
  • Further clinical validation of POC systems for RPR monitoring is essential.
Abstract

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