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Related Experiment Videos

Duplexed sandwich immunoassays on a fiber-optic microarray.

David M Rissin1, David R Walt

  • 1Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, MA 02155, USA.

Analytica Chimica Acta
|August 29, 2007
PubMed
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This study presents a novel fiber-optic array immunoassay for simultaneously detecting immunoglobulin A (IgA) and lactoferrin in saliva. The developed platform shows potential for increased multiplexing in diagnostic applications.

Area of Science:

  • Biomedical Engineering
  • Immunotechnology
  • Analytical Chemistry

Background:

  • Innates immune system proteins like immunoglobulin A (IgA) and lactoferrin are crucial biomarkers in saliva.
  • Simultaneous detection of multiple biomarkers requires advanced analytical platforms.
  • Existing immunoassay methods may have limitations in sensitivity or multiplexing capabilities.

Purpose of the Study:

  • To develop and characterize a duplexed, fiber-optic array-based immunoassay.
  • To enable simultaneous detection of IgA and lactoferrin in human saliva.
  • To assess the performance and potential for multiplexing of the developed platform.

Main Methods:

  • Fabrication of an individually addressable optical fiber array using functionalized microspheres.

Related Experiment Videos

  • Loading of antibody-coated microspheres into microwells on an imaging optical fiber bundle.
  • Development of two sandwich immunoassays for simultaneous detection of IgA and lactoferrin.
  • Characterization of working concentration ranges and cross-reactivity.

    • Main Results:

    • The immunoassay successfully detected both IgA (700 pM - 100 nM) and lactoferrin (385 pM - 10 nM) simultaneously.
    • Low cross-reactivity was observed between detection antibodies and non-specific targets.
    • Individual microspheres allowed for interrogation of both analytes.

    Conclusions:

    • The developed fiber-optic array immunoassay is capable of sensitive and specific simultaneous detection of IgA and lactoferrin.
    • The platform demonstrates potential for higher degrees of multiplexing beyond duplex detection.
    • This technology offers a promising approach for advanced salivary diagnostics.