Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simplified competitive protein-binding assay for 25-hydroxycalciferol.

B Garcia-Pascual, A Peytremann, B Courvoisier

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |April 15, 1976
    PubMed
    Summary

    A new protein-binding assay accurately measures plasma 25-hydroxyvitamin D (25-OH-D) without chromatography or beta-lipoproteins. This method is reliable for clinical studies, revealing lower 25-OH-D levels in alcoholic patients.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Infection prevention and control measures in practices of the Swiss sentinel network during seasonal influenza epidemics.

    The Journal of hospital infection·2020
    Same author

    When adoption is in question.

    Modern hospital·2010
    Same author

    Stabilizing feedbacks in glacier-bed erosion.

    Nature·2003
    Same author

    Short- and long-term results of total vs subtotal thyroidectomies in the surgical treatment of Graves' disease.

    Swiss surgery = Schweizer Chirurgie = Chirurgie suisse = Chirurgia svizzera·2001
    Same author

    [Ambulatory surgery in a private clinic].

    Revue medicale de la Suisse romande·1998
    Same author

    A recommendation on recommendations.

    AJR. American journal of roentgenology·1997

    Area of Science:

    • Clinical Chemistry
    • Endocrinology
    • Nutritional Science

    Background:

    • Accurate measurement of plasma 25-hydroxyvitamin D (25-OH-D) is crucial for assessing vitamin D status.
    • Existing assays often involve complex steps like chromatography and may be affected by beta-lipoproteins.
    • A simplified, reliable assay is needed for widespread clinical application.

    Purpose of the Study:

    • To evaluate a simplified protein-binding assay for plasma 25-OH-D.
    • To assess the assay's performance without chromatographic separation and in the absence of beta-lipoproteins.
    • To determine plasma 25-OH-D levels in healthy volunteers and alcoholic patients.

    Main Methods:

    • A competitive protein-binding assay was adapted, omitting the chromatographic step.

    Related Experiment Videos

  • Assay accuracy and precision were assessed with and without beta-lipoproteins.
  • Plasma 25-OH-D levels were measured in healthy adults and patients with alcoholism.

    • Main Results:

    • The assay demonstrated similar accuracy with or without beta-lipoproteins (110-136% vs. 74-85% recovery).
    • Intra-assay variation was lower without beta-lipoproteins (7.23% vs. 12.56%).
    • Mean plasma 25-OH-D in healthy volunteers was 39.5 ± 9.3 µg/l; alcoholic patients showed significantly lower levels (13.0 ± 8.9 µg/l).

    Conclusions:

    • The described protein-binding assay is precise, accurate, and convenient for clinical studies.
    • Eliminating chromatography and beta-lipoproteins simplifies the measurement of plasma 25-OH-D.
    • Alcoholism is associated with reduced plasma 25-OH-D levels.