Automated sequential injection fluorimetric set-up for multiple release testing of topical formulation.

Area of Science:

• Analytical Chemistry
• Pharmaceutical Science
• Drug Delivery Systems

Background:

• Ointment formulations require robust methods for drug release testing.
• Simultaneous analysis of multiple samples can improve efficiency in pharmaceutical quality control.

Purpose of the Study:

• To develop and validate a fully automated sequential injection analysis (SIA) device for simultaneous release testing of multiple ointment samples.
• To apply the developed SIA system for the release testing of a salicylic acid ointment.

Main Methods:

• A sequential injection analysis (SIA) system was designed, incorporating a syringe pump, selection valves, peristaltic pump, and fluorescence detector.
• Ointment samples were placed on poly(carbonate) membranes within Franz cells containing phosphate buffer (pH 7.4).
• Acceptor liquid samples were automatically aspirated, analyzed for salicylic acid concentration via fluorescence detection, and replenished over a 6-hour period.

Main Results:

• The SIA system successfully performed simultaneous release testing of up to six ointment samples.
• A linear calibration curve for salicylic acid was established in the range of 1-100 µg/mL.
• The device facilitated automated, computer-aided evaluation of drug release profile parameters.

Conclusions:

• The developed automated SIA device is effective for simultaneous drug release testing of ointments.
• This method offers an efficient and reliable approach for quality control of topical formulations.
• The system allows for precise monitoring and analysis of drug release kinetics.