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Related Concept Videos

Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Drug Absorption Mechanism: Passive Membrane Transport01:23

Drug Absorption Mechanism: Passive Membrane Transport

Passive transport is a method of drug absorption where small, lipid-soluble drugs can move across the cell membrane. This movement happens along the concentration gradient, which is a natural flow from higher to lower concentration areas. The speed at which the drug moves is directly related to its lipid–water partition coefficient. This means that the more a drug dissolves in lipids, the faster it diffuses or spreads throughout the body. It is important to note that most drugs are either weak...

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Related Experiment Video

Updated: Jul 12, 2026

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

Cadmium bioavailability and speciation using the permeation liquid membrane.

Stéphane Bayen1, Isabelle Worms, Nalini Parthasarathy

  • 1CABE, University of Geneva, Sciences II, 30 Quai Ernest Ansermet, CH 1211, Geneva 4, Switzerland. stephane.bayen@cabe.unige.ch

Analytica Chimica Acta
|August 29, 2007
PubMed
Summary

The permeation liquid membrane (PLM) technique accurately measures free cadmium (Cd) in freshwater, aiding in understanding its bioavailability to aquatic organisms like algae. This method shows promise for predicting how much cadmium is taken up by living cells.

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Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films
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Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films

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Last Updated: Jul 12, 2026

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films
12:22

Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films

Published on: November 9, 2015

Area of Science:

  • Environmental Chemistry
  • Aquatic Toxicology
  • Analytical Chemistry

Background:

  • Cadmium (Cd) speciation in natural waters is complex due to various ligands.
  • Accurate measurement of free Cd2+ is crucial for assessing its environmental risk and bioavailability.
  • Existing methods for Cd speciation may have limitations in complex natural matrices.

Purpose of the Study:

  • To evaluate the permeation liquid membrane (PLM) technique for determining cadmium speciation in freshwater media.
  • To compare PLM measurements of free Cd with theoretical calculations and selective electrode data.
  • To assess the utility of PLM as a predictor of cadmium bioavailability to freshwater algae.

Main Methods:

  • Characterization of a planar sheet PLM system by measuring Cd fluxes with and without complexing agents (citrate, malonate, NTA, humic acid).
  • Comparison of PLM results with theoretical speciation calculations and Cd2+ selective electrode measurements.
  • Investigation of pH and co-transported ion effects on Cd transport through PLM.
  • Evaluation of hollow-fiber PLM (HFPLM) for measuring nanomolar free Cd.
  • Comparison of Cd PLM fluxes with Cd biouptake (internalization flux) in Chlorella kesslerii.

Main Results:

  • The planar sheet PLM accurately measured free Cd within the 10(-8) to 10(-4) M concentration range.
  • PLM measurements correlated well with theoretical speciation and selective electrode data.
  • The effect of pH and co-transported ions on Cd transport was elucidated.
  • HFPLM demonstrated capability for measuring nM range free Cd.
  • Cd PLM fluxes showed a relationship with Cd biouptake in Chlorella kesslerii, especially in the presence of humic acid.

Conclusions:

  • The permeation liquid membrane technique is a reliable method for quantifying free cadmium in freshwater environments.
  • PLM offers a valuable tool for understanding cadmium speciation and its influence on bioavailability.
  • PLM measurements can serve as a predictive indicator of cadmium uptake by aquatic organisms, improving ecological risk assessments.