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Surface plasmon resonance immunosensor for histamine based on an indirect competitive immunoreaction
Yan Li1, Masatoshi Kobayashi, Koji Furui
1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Analytica Chimica Acta
|August 29, 2007
Summary
This study introduces a reusable surface plasmon resonance immunosensor for histamine detection. The novel method achieves a low detection limit of 3ppb for histamine analysis.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Immunosensors
Background:
- Histamine (beta-imidazole ethylamine) analysis is crucial in various fields.
- Existing methods for histamine detection may have limitations in sensitivity or reusability.
- Development of rapid and sensitive immunosensors is an active area of research.
Purpose of the Study:
- To develop and characterize a surface plasmon resonance (SPR) immunosensor for histamine detection.
- To evaluate the performance and reusability of the developed histamine immunosensor.
- To determine the detection limit and affinity constants of the SPR immunosensor.
Main Methods:
- Fabrication of a sensor chip with immobilized histamine using 11-mercaptoundecanoic acid (11-MUA) self-assembly monolayer and amino-coupling.
- Utilized an indirect competitive immunoassay format with anti-histamine antibodies.
- Regeneration of the sensor chip with 10mM HCl for reusability.
Main Results:
- The SPR immunosensor demonstrated a detection limit of 3ppb for histamine.
- Calculated affinity constants for the immunocomplexes were 1.5 x 10(7) M(-1) and 7.2 x 10(5) M(-1).
- The sensor chip showed reusability after regeneration with a 10mM HCl solution.
Conclusions:
- A sensitive and reusable SPR immunosensor for histamine analysis has been successfully developed.
- The indirect competitive assay format provides a viable approach for histamine detection.
- The developed method offers potential for rapid and efficient histamine quantification.
