Sensors based on galvanic cell generated electrochemiluminescence and its application

Area of Science:

• Analytical Chemistry
• Electrochemistry
• Biosensors

Background:

• Hydrogen peroxide (H2O2) is a key analyte in various biological and environmental processes.
• Existing detection methods for H2O2 can be limited by sensitivity, stability, or cost.
• Electrochemical methods offer promising alternatives for sensitive and selective analyte detection.

Purpose of the Study:

• To develop a novel electrochemiluminescence (ECL) imaging sensor array for hydrogen peroxide determination.
• To utilize a Cu/Zn alloy galvanic cell for stable potential generation in ECL assays.
• To enhance weak ECL emission using H2O2 for improved detection.

Main Methods:

• Fabrication of a galvanic cell sensor array by immobilizing Cu/Zn alloy in 96-well microtiter plates.
• Utilizing the corrosion effect in alkaline solution to form a stable Cu/Zn galvanic cell.
• Employing luminol as the ECL emitter, enhanced by H2O2, for quantitative analysis.

Main Results:

• The ECL intensity showed a linear relationship with H2O2 concentration from 1.0 x 10(-6) to 1.0 x 10(-4) mol L(-1).
• A low detection limit of 3.0 x 10(-7) mol L(-1) (3sigma) was achieved for H2O2.
• The sensor array demonstrated good precision with a relative standard deviation (R.S.D.) of 4.0% for 1.0 x 10(-5) mol L(-1) H2O2.

Conclusions:

• The developed Cu/Zn alloy galvanic cell-based ECL sensor array is effective for sensitive H2O2 determination.
• The sensor offers a stable and enhanced ECL signal, suitable for quantitative analysis.
• This novel sensor platform holds potential for H2O2 detection in various applications.